|HORN, PATRICK - University Of North Texas|
|BLASZCYK, SYLVIA - University Of North Texas|
|NEOGI, PURNIMA - University Of North Texas|
|TOMBOKAN, XENIA - Bruker Optics|
|GHOSH, SUPRIYO - University Of North Texas|
|Campbell, Benjamin - Todd|
|CHAPMAN, KENT - University Of North Texas|
Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/17/2011
Publication Date: 1/24/2011
Citation: Horn, P., Blaszcyk, S., Neogi, P., Tombokan, X., Ghosh, S., Campbell, B.T., Chapman, K. 2011. Improved quantification of cottonseed protein and oil content for germplasm screening. In: Proceedings of the National Cotton Council Beltwide Cotton Conference, January 4-7, 2011, Atlanta, Georgia. CDROM.
Technical Abstract: Oil and protein stores comprise the majority of mass of cottonseed embryos. A more comprehensive understanding of the relationship between lint quality, lint yield and embryo reserve accumulation will help assist breeders in their efforts to improve seed value. Here we report the improvement of a rapid, non-destructive, simultaneous method for quantifying oil and protein levels within cottonseed by low-field, time-domain 1H nuclear magnetic resonance (TD-NMR). This approach is suitable for a minimal amount of seed and represents an accurate (oil R2 = 0.998, protein R2 = 0.97), non-invasive alternative to conventional, time consuming chemical extractions. We demonstrate the value of this approach by surveying seed reserve content, identifying extremes of either protein and/or oil, in two sets of diverse germplasm.