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United States Department of Agriculture

Agricultural Research Service

Research Project: SIV EPITOPES AND GENETIC FACTORS THAT AFFECT SURVEILLANCE AND IMMUNITY ELICITED BY INFLUENZA VACCINES AGAINST EMERGING INFLUENZA STRAINS

Location: Virus and Prion Research

Title: Comparison of Humoral and Cellular Immune Responses to Inactivated Swine Influenza Virus Vaccine in Weaned Pigs)

Author
item Platt, Ratree
item Gauger, Phillip
item Kimura, Kayoko
item Roth, James
item Vincent, Amy
item Loving, Crystal
item Zanella, Eraldo
item Lager, Kelly
item Kehrli Jr, Marcus

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/30/2010
Publication Date: 12/7/2010
Citation: Platt, R., Gauger, P., Kimura, K., Roth, J., Vincent, A., Loving, C., Zanella, E., Lager, K., Kehrli, M. 2010. Comparison of humoral and cellular immune responses to inactivated swine influenza virus vaccine in weaned pigs [abstract]. Conference of Research Workers in Animal Diseases. Paper No. 085P/85P.

Interpretive Summary:

Technical Abstract: Purpose: To evaluate and compare humoral and cellular immune responses to inactivated swine influenza virus (SIV) vaccine. Methods: Fifty 3-week-old weaned pigs from a herd free of SIV and PRRSV were randomly divided into the non-vaccinated control group and vaccinated group containing 25 pigs each. Pigs were vaccinated intramuscularly twice with adjuvanted UV-inactivated A/SW/MN/02011/08 (MN/08) H1N1 SIV vaccine at 6 and 9 weeks of age. Whole blood samples for multi-parameter flow cytometry (MP-FCM) and serum samples for hemagglutination inhibition (HI) assay were collected at 23 and 28 days after the second vaccination respectively. A standard HI assay and MP-FCM were performed against UV-inactivated homologous MN/08 and heterologous pandemic A/CA/04/2009 H1N1 (CA/09) viruses. Results: While the HI assay detected humoral responses only to the MN/08 virus, the MP-FCM detected strong cellular responses against the MN/08 delta-cluster virus and lower but significant heterologous responses to the CA/09 gamma-cluster virus, especially in the CD4+CD8+ T cell subset. Conclusions: The cellular heterologous responses to UV-inactivated gamma-cluster virus by MP-FCM suggested that the assay was sensitive and potentially detected a wider range of antigens than what is detected by the HI assay. The MP-FCM also identified the responses of multiple parameters in different T cell subsets. Overall, the adjuvanted UV-inactivated A/SW/MN/02011/08 H1N1 SIV vaccine stimulated both humoral and cellular immune responses including the CD4-CD8+ T cell subset.

Last Modified: 8/24/2016
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