Effects of dietary fat on spontaneous metastasis of Lewis lung carcinoma and changes in plasma cytokine concentrations in mice

Authors: Yan, Lin

Submitted to: American Association of Cancer Research Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 7/10/2010
Publication Date: 9/15/2010
Citation: Yan, L. 2010. Effects of dietary fat on spontaneous metastasis of Lewis lung carcinoma and changes in plasma cytokine concentrations in mice. American Association of Cancer Research Meeting. Abstract No.B41 p.107.

Interpretive Summary:

Technical Abstract: The present study assessed the effects of dietary fat on spontaneous metastasis of Lewis lung carcinoma in mice. Three-week old male C57BL/6 mice were fed the AIN-93G standard diet or a 45% fat diet (kcal %) for seven weeks before they were subcutaneously injected with 2.5 x 105 viable cells into the lower dorsal region. The primary tumor was resected two weeks later, and mice were maintained on their respective diets for additional two weeks before the termination. Feeding mice the high-fat diet significantly increased body weight and abdominal adipose content compared to the AIN-93G standard diet. There was a 2-fold increase in the number of tumor nodules developed in the lungs in the high-fat diet-fed mice compared to the standard diet-fed mice (P<0.05). There were no significant differences in plasma concentrations of TIMP-1, IL-1ß, VEGF and MCP-1 in non-tumor bearing mice fed the standard diet or the high-fat diet (referred to as control baseline and high-fat diet baseline). There were 3-, 4-, 40- and 12-fold increase in plasma TIMP-1 (non-significant), IL-1ß (P<0.05), VEGF (P<0.05) and MCP-1 (P<0.05) in tumor-bearing mice fed the standard diet compared to the control baseline. Dietary supplementation with 45% fat further significantly increased these cytokines in tumor-bearing mice (P<0.05 for each comparison). Feeding mice (either tumor-bearing or non-tumor-bearing) the high-fat diet significantly increased plasma concentration of leptin and significantly decreased plasma adiponectin compared to the standard diet. Results of the present study demonstrated that the high-fat diet enhanced spontaneous metastasis of Lewis lung carcinoma in mice and that this aggressiveness was accompanied with significant increases in plasma.