Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2009
Publication Date: 4/24/2010
Publication URL: http://www.fasebj.org
Citation: Picklo, M.J., Idso, J.P. 2010. Obesity Induces Tissue-Specific Changes in Lipid Peroxidation Defense Enzymes. Journal of Federation of American Societies for Experimental Biology. 24:547.9. Interpretive Summary:
Technical Abstract: Lipid peroxidation is thought to be a component of obesity-induced pathology. However, the tissue-dependent changes in lipid peroxidation (LOOH) and LOOH defense mechanisms in response to obesity are unclear. In this work, we utilized 14-week old male, obese Zucker rats and their control, lean littermates and measured levels of LOOH and LOOH defense enzymes in liver, visceral (peri-renal) fat and sub-cutaneous fat. Hepatic 4-hydroxy-2-nonenal (HNE) protein adducts (a measure of LOOH) were elevated (12%) in the obese rats versus controls; whereas obesity significantly decreased HNE protein adducts (54%) in visceral fat. There was no effect of obesity upon the activities of the LOOH defense enzymes glutathione peroxidase, glutathione S-transferase, and mitochondrial class 2 and 5 aldehyde dehydrogenases in these tissues. On the other hand, class 3 aldehyde dehydrogenase activity was significant decreased (23%) in liver and adipose depots (>80%) in the obese rats. Specific activities of all LOOH defense enzymes were higher in liver than in adipose tissues in lean and obese animals. These data demonstrate that obesity has tissue-dependent effects upon lipid peroxidation and LOOH inhibitory enzymes.