Submitted to: Journal of Animal Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/28/2009
Publication Date: 1/1/2010
Publication URL: http://handle.nal.usda.gov/10113/39844
Citation: Rempel, L.A., Nonneman, D.J., Wise, T.H., Erkens, T., Peelman, L.J., Rohrer, G.A. 2010. Association Analyses of Candidate Single Nucleotide Polymorphisms on Reproductive Traits in Swine. Journal of Animal Science. 88:1-15. Interpretive Summary: Results of this study indicate that genetic factors impact reproductive and production traits in a Landrace-Duroc-Yorkshire swine population potentially influencing sow longevity. The use of genetic marker association analyses provides direction or gives an indication as to where potential quantitative trait loci reside. Within this study we found that several of the candidate markers were associated with metabolic parameters indicating energy demands of first parity gilts may play a large role in successful return to service. Furthermore, this information may lead to fine mapping of regions of the DNA that will give insight into possible nucleotides and/or genes that impact sow productive lifetime potential that could be used for selection assistance of young females prior to entry within the breeding herd.
Technical Abstract: The ability to identify young females with superior reproduction would have a large economic impact on commercial swine production. Previous studies have discovered SNP associated with economically important traits such as litter size, growth rate, and feed intake. The objective of this study was to test for association candidate SNP with sow prolificacy production traits in gilts of a Landrace-Duroc-Yorkshire composite population. Association analyses regressed additive (A), dominant (D), and imprinting (I) SNP effects on each trait with an animal model. A carnitine palmitoyltransferase 1A SNP and a glycogen synthase 1 SNP were associated with age at puberty (AP; D = 10 d; P = 0. 0037 and A = 3.8 d; P = 0.0078, respectively). Four IGF2 SNP were associated with AP as well, having additive or dominant effects (3.2 to 5.8 d; P < 0.0052). Two mannosidase 2B2 SNP and two prolactin receptor (PRLR) SNP were also associated with AP. Solute carrier 22, subfamily member 5 SNP was weakly associated with AP (D = 3.9 d; P < 0.10). Polymorphisms within glycogen synthase 1 and protein kinase AMP-activated, gamma 3 non-catalytic subunit had associations with ovulation rate. Estrogen receptor (ESR) 1, ESR2, peroxisome proliferator-activated receptor, gamma, coactivator 1, and IGFBP3 SNP were significantly associated with weaning-to-estrus interval. Two PRLR SNP were associated with total number of piglets born (A = 0.57 piglets; P = 0.0095 and D = 0.61 piglets; P = 0.0016, respectively). A SNP within PRLR was also associated with number of piglets born alive (D = 0.61; P = 0.0016). Peroxisome proliferator-activated receptor, gamma, coactivator 1 SNP was associated with total number of piglets born (D = 0.38 piglets; P = 0.0391) and number of piglets born alive (D = 0.53 piglets; P = 0.0032). SNP within ESR1 (A = 0.65 piglets; P =0.0950), ESR2 (A = -0.33 piglets; P = 0.0176), IGF2 SNP (A = -0.26 piglets; P = 0.0032), and IGFBP3 SNP (D = 0.35 piglets; P = 0.0683) were associated with number of piglets born dead. A leptin SNP was associated with mummified fetuses (D = 0.09 piglets; P = 0.0978). Many of the SNP analyzed in this study are from genes involved in regulation of metabolism suggesting that there is an important link between physiological events associated with reproduction and energy utilization. Furthermore these production and growth trait SNP may serve to assist in selection of young females for superior reproductive performance.