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Title: Design of the Illumina Porcine 50K+ SNP Iselect(TM) Beadchip and Characterization of the Porcine HapMap Population

item GROENEN, MARTIEN - Wageningen University
item CROOIJMANS, RICHARD - Wageningen University
item RAMOS, ANTONIO - Wageningen University
item AMARAL, ANDREIA - Wageningen University
item KERSTENS, HINRI - Wageningen University
item BENDIXEN, CHRISTIAN - University Of Aarhus
item HEDEGAARD, JACOB - University Of Aarhus
item Rohrer, Gary
item Smith, Timothy - Tim
item Van Tassell, Curtis - Curt
item TAYLOR, JEREMY - University Of Missouri
item ROTHSCHILD, MAX - Iowa State University
item HU, ZHILIANG - Iowa State University
item Nonneman, Danny - Dan
item BEEVER, JON - University Of Illinois
item ARCHIBALD, ALAN - Roslin Institute
item LAW, ANDY - Roslin Institute
item MILAN, DENIS - Institut National De La Recherche Agronomique (INRA)
item HANSEN, MARK - Illumina, Inc
item SCHOOK, LARRY - University Of Illinois

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2008
Publication Date: 1/10/2009
Citation: Groenen, M.A., Crooijmans, R.P., Ramos, A.M., Amaral, A.J., Kerstens, H., Bendixen, C., Hedegaard, J., Rohrer, G.A., Smith, T.P., Van Tassell, C.P., Taylor, J.F., Rothschild, M., Hu, Z., Nonneman, D.J., Beever, J., Archibald, A., Law, A., Milan, D., Hansen, M., Schook, L. 2009. Design of the Illumina Porcine 50K+ SNP Iselect(TM) Beadchip and Characterization of the Porcine HapMap Population. Plant and Animal Genome Conference Proceedings. 1/10-14/09, San Diego, CA. Abstract W493.

Interpretive Summary:

Technical Abstract: Using next generation sequencing technology the International Swine SNP Consortium has identified 500,000 SNPs and used these to design an Illumina Infinium iSelect™ SNP BeadChip with a selection of 60,218 SNPs. The selected SNPs include previously validated SNPs and SNPs identified de novo using second generation sequencing on the Illumina Genome Analyzer (Solexa) and Roche 454 FLX sequencer. Towards this end, 16 reduced representation libraries (RRL) were prepared using pooled DNA samples from individuals from five breeds (Duroc, Landrace, Large White, Pietrain, Wild Boar) digested with four restriction enzymes (AluI, HaeIII, MspI and DraI). Fragments in the size range of 150-200 bp were selected for sequencing. The ends of these fragments (36 bp) were sequenced to a 30X coverage on the Genome Analyzer and in case of the AluI libraries complemented with 2X sequencing of the complete fragment using 454 technology. For 41,667 SNPs coordinates on genome build 7, representing approximately 70 % of the porcine genome are available. Of the remaining 18,545 SNPs provisional predicted positions on the porcine genome based on the human-pig comparative map are available for 4,497 SNPs. The BeadChip was used to genotype a panel of 576 individuals representing the original discovery panel as well as representative trios from a variety of pig breeds. First results on the validation of the SNPs, linkage disequilibrium and breed characteristics will be presented.