Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 1/7/2009
Publication Date: 4/27/2009
Publication URL: http://www.fasebj.org
Citation: Combs, G.F., Zeng, H., Jackson, M.I., Johnson, L.K., Hoeg, A., Schomburg, L., Davis, C.D., Milner, J.A. 2009. Genetic Determinants of Responses to Selenium Supplementation. Journal of Federation of American Societies for Experimental Biology. 23:346.3. Interpretive Summary:
Technical Abstract: In a cohort of healthy adults (106 M, 155 W) in eastern North Dakota, we determined the relationships of five biomarkers of selenium (Se) status (plasma Se, serum selenoprotein P [SePP], plasma glutathione peroxidase [GPX3] activity, buccal cell Se, urine Se) to genotype for four selenoproteins (cytosolic glutathione peroxidase [GPX1], phospholipid hydroperoxide glutathione peroxidase [GPX4], the 15 kD selenoprotein [SeP15], SePP) and two glutathione S-transferases (GST-M1, GST-T1). The cohort had plasma Se of 141.5±23.7 (SD) ng/ml, SePP lof 3.55 (CI 2.61, 4.51) mg/L, GPX3 activities of 3.64±0.54 nmoles NADPH/min/mg protein, buccal cell Se of 10.2±6.5 ng Se/mg protein, and urinary Se of 58.2±21.5 ng Se/mg creatinine. Plasma Se was significantly related to GPX3 genotype (198Leu/Leu: 135.7a±19.0 ng/ml; 198Leu/Pro: 139.2a,b±23.8 ng/ml; 198 Pro/Pro: 145.9b±24.4 ng/ml; P<0.05). Buccal cell Se was significantly related to SeP15 genotype (C/C: 8.77a [5.85, 13.14]; T/C: 7.65b [5.06, 11.57]; T/T: 8.27a,b [6.16, 11.10] P<0.05). SePP was significantly related to SePP genotype at a polymorphism in the 3’-UTR region (A/A: 3.49a,b [2.91, 4.18]; G/A: 3.24 a [2.44, 4.31]; G/G: 3.62 b [2.75, 4.76]), but not one in the coding region (A234T). No other effects were detected. These results show that genetic polymorphisms can significantly affect some biomarkers used to assess Se status.