Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: 10/10/2008
Publication Date: 10/13/2008
Citation: Yeh, H., Klesius, P.H. 2008. Channel Catfish, Ictalurus punctatus, ubiquitin carboxy-terminal hydrolase L5 cDNA. Genbank. Accession No. FJ205474.
Technical Abstract: The ubiquitin-proteasome cycle is a complex, non-lysosomal biochemical process for intracellular protein degradation. This process involves many enzymes. One of them is ubiquitin carboxy-terminal hydrolase (UCT). In this report, we cloned, sequenced and characterized the channel catfish UCT L5 cDNA. The complete nucleic acid sequence of channel catfish UCT L5 cDNA comprised of 1,357 nucleotides, including an open reading frame, which appears to encode a putative peptide of 329 amino acid residues. The estimated molecular mass and pl of this peptide are 37.6 kDa and 4.84, respectively. The degree of conservation of the channel catfish UCT L5 amino acid sequence in comparison to other species ranged from 79% to 92%. The channel catfish UCT L5 transcript was detected by RT-PCR in spleen, head kidney, liver, intestine, skin and gill. This result provides important information for further elucidating UCT L5 in channel catfish.