Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/2008
Publication Date: 1/10/2009
Citation: Chiang, H., Swaggerty, C.L., Kogut, M.H., Zhou, H. 2009. Gene expression profiling in Salmonella enterica serovar Typhimurium stimulated heterophils using a chicken 44k agilent microarray [abstract]. Plant & Animal Genome XVII Conference, January 10-14, 2009, San Diego, California. p. 513.
Technical Abstract: Microarray technology is a powerful high-throughput tool for the analysis of host-pathogen interactions that permits ascribing the transcriptional status of thousands of genes simultaneously. To determine the host response to Salmonella Typhimurium (ST) in heterophils, a whole chicken genome array was used to analyze RNA isolated from heterophils and then stimulated in vitro with ST (30 min, 1 h, 2 h, and 3 h) or medium control (NS). A dual-color balanced design was used to provide a direct comparison between ST-stimulated and non-stimulated controls (30 min vs. NS (30M/NS), 1 h vs. NS (1HR/NS), 2 h vs. NS (2HR/NS) and 3 h vs. NS (3HR/NS)). Each comparison includes four biological replicates. There were 2791, 3744, 12947, and 12482 genes differentially expressed in the comparisons of 30M/NS, 1HR/NS, 2HR/NS and 3HR/NS, respectively (P less than 0.001), while 50, 82, 153, and 146 genes were related to immune function, respectively. More than 80% of immune-related genes overlapped between the comparisons (30M/NS vs. 1HR/NS, 1HR/NS vs. 2HR/NS, and 2HR/NS vs. 3HR/NS). These candidate genes include cytokines [e.g. Interleukin (IL) 1beta, -6, -10B and -12B], chemokines (e.g. IL-8, CCL4, K60, and K203), and cluster of differentiation (CD) markers (e.g. CD44, -47 and -83). A relatively large increase in the number of differentially expressed immune-related genes was observed between 1 h and 2 h post-stimulation. The results of continuous transcriptional change discovered in the present study provide important information to elucidate both the cellular and molecular mechanisms of ST infection in chickens.