|Nonneman, Danny - Dan|
Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2008
Publication Date: 1/10/2009
Citation: Lindholm-Perry, A.K., Rohrer, G.A., Keele, J.W., Holl, J.W., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M., Nonneman, D.J. 2009. Positional Candidate Genes for Kyphosis in Commercial Swine Populations. Proc., Plant and Animal Genome Conference XVII. 1/10-14/2009, San Diego, CA. P529.
Technical Abstract: A back curvature defect similar to kyphosis in humans has been observed in swine herds. The defect ranges from mild to severe curvature of the thoracic vertebrate in split carcasses and has an estimated heritability of 0.3. The purpose of this study was to identify genomic regions that affect this trait. The R program QTLbim was used to detect regions with main effects and epistatic interactions for back curvature in a Duroc-Landrace F2 discovery population. QTL regions were detected on chromosomes SSC1, SSC5 and SSC6 suggesting that this back curvature trait is affected by several loci. Positional candidate genes that play a role in human skeletal development pathways were selected for single nucleotide polymorphism (SNP) identification. Thirty SNP were genotyped in an unrelated Duroc-Landrace-Yorkshire population, with a total of 1216 animals phenotyped for the back curvature defect. The QTL association option of the statistical analysis program Mendel was used to test whether SNP in candidate genes were associated with back curvature. SNP in CER1 and PLOD1 located on chromosomes 1 and 6, respectively, were significantly associated with the back curvature trait (p-values - 0.01). The genes identified in this study may affect skeletal characteristics in swine. Carcass value and cutability could be improved by effectively removing this undesirable trait from pig populations.