Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/14/2008
Publication Date: 12/5/2008
Citation: Miller, L.C., Chitko-Mckown, C.G., Lager, K.M., Kehrli, Jr., M.E. 2008. Differential Roles of Toll-Like Receptors in the Elicitation of Type I Interferon Responses by Alveolar Macrophages [abstract]. International PRRS Symposium. p. 58.
Technical Abstract: Control of virus replication initially depends on rapid activation of the innate immune responses. Toll-like receptor (TLR) ligands are potent inducers of innate immunity against viral infections. Porcine reproductive and respiratory syndrome virus (PRRSV) initiates infection in pulmonary alveolar macrophages (PAMs), elicits weak immune responses, and establishes a persistent infection. To understand the role of ssRNA and dsRNA intermediates in eliciting host immunity, we sought to determine if Toll-like receptors (TLRs), particularly those that respond to viral molecular patterns, are involved in PRRSV infection. Activation of TLR-3 in PAMs with dsRNA, increased gene expression for interferon-alpha and suppressed PRRSV infectivity. In contrast, treating PAMs with LPS for TLR-4 did not influence PRRSV infectivity.