Submitted to: American Chemical Society Midwest Regional Meeting
Publication Type: Abstract only
Publication Acceptance Date: 9/15/2008
Publication Date: 9/15/2008
Citation: Tabatabai, L.B., Zimmerli, M., Zehr, E.S. 2008. Strategies for Development of a Peptide Vaccine for Poultry Respiratory Disease [abstract]. American Chemical Society Midwest Regional Meeting, October 5-8, 2008, Kearney, Nebraska. Paper No. 95. Interpretive Summary:
Technical Abstract: Bacterial respiratory disease of turkeys causes millions of dollars in economic losses to the poultry industries. Poultry or avian respiratory disease complex may involve several pathogens both viral and bacterial, and disease is exacerbated by environmental stress. Live attenuated vaccines are available for some of these pathogens. However, it would be preferable if specific, multivalent, cell-free, and stable vaccines could be used. One such approach is the rational development of peptide vaccines. Peptide vaccines, by design, would only display the necessary epitopes and be devoid of unnecessary polypeptide “baggage”. The strategy adapted in our laboratory is the following: 1) identify immunoreactive epitopes on a Western blot; 2) identify the corresponding proteins on a stained companion 2-D gel; 3) excise spot, digest with trypsin, run a peptide mass fingerprint, identify homolog or orthologs; 4) if the genome is not in the database perform LC-MS/MS peptide sequencing; 5) identify the immunoreactive epitopes and synthesize the corresponding peptides with a N-terminal cysteine for coupling to a carrier protein if needed. Preliminary immunization experiments using such peptide vaccines are currently being evaluated in turkeys.