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ARS Home » Plains Area » Bushland, Texas » Conservation and Production Research Laboratory » Livestock Nutrient Management Research » Research » Publications at this Location » Publication #229202

Title: Removal of endotoxin from dairy wastewater

item Purdy, Charles

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/21/2008
Publication Date: 12/7/2008
Citation: Purdy, C.W., Straus, D. 2008. Removal of endotoxin from dairy wastewater [abstract]. In: Proceedings of 89th Annual Meeeting of Conference of Research Workers in Animal Diseases, December 7-9, 2008, Chicago, Illinois. No. 139. p.155.

Interpretive Summary:

Technical Abstract: The efficacy of various treatments on removing endotoxin (ET) from wastewater was tested by using the treated water to induce a systemic reaction via intratracheal inoculation (20 ml/goat, 6 goats/group). Treatments (T1-T7) of wastewater were as follows: 1) autoclaved 15 min, centrifuged and contained 17.56 ug ET/20 ml.; 2) treated once with calcium silicate hydrate (LRA) (15g/L), centrifuged, sterile filtered, contained 0.962 ug ET/20 ml.; 3) treated once with LRA, centrifuged, sterile filtered and ET replaced with known E. coli ET, contained 16.60 ug/20 ml.; 4) treated 5 times with LRA, autoclaved 10 times, frozen and thawed 3 times, centrifuged, sterile filtered, contained 3.12 ug ET/20 ml.; 5) treated 5 times with LRA, centrifuged, sterile filtered, contained 2.72 ug ET/20 ml.; 6) sterile ET free water; and 7) pen controls, no treatments. Systemic reactions were monitored by rectal temperatures (RT) and total white blood cell (WBC) counts. Results were as follows: T1 and T3 were not significantly different and gave a typical ET systemic response (> rectal temperature and total WBC). T6 and T7 had no ET temperature response. T2 and T5 had shorter (>3-4 hr RT) ET response, and T4 was similar to the T6 and T7 RT response, except for slight increases in RT at 6 and 12 hrs. These results suggest an ET dose of 0.9 to 3.12 ug induced an aberrant in vivo systemic response