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ARS Home » Southeast Area » Mayaguez, Puerto Rico » Tropical Crops and Germplasm Research » Research » Publications at this Location » Publication #226095
Title: MUTAGENESIS OF BAT 93 FOR TILLING IN COMMON BEAN

Author
item Porch, Timothy - Tim
item BLAIR, M. - CIAT, COLOMBIA
item LARIGUET, P. - UNIVERSITY OF GENEVA
item BROUGHTON, W - UNIVERSITY OF GENEVA

Submitted to: Bean Improvement Cooperative Annual Report
Publication Type: Research Notes
Publication Acceptance Date: 3/1/2008
Publication Date: 3/31/2008
Citation: Porch, T.G., Blair, M., Lariguet, P., Broughton, W. 2008. Mutagenesis of bat 93 for tilling in common bean. Bean Improvement Cooperative Annual Report. 51:18-19.

Interpretive Summary: An important means for the study of agriculturally important traits is through the generation of mutations in specific genes of interest. This study optimized the concentration of the mutagen ethyl methanesulfonate (EMS) for use in the development of a large population of mutants in BAT 93, a bean variety. Because there are few mutants available in common bean, the population will represent an important resource for genetic analysis of any gene in the genome by enabling the identification of mutations in specific genes using TILLING (targeting induced local lesions in genomes). In this study, genotype BAT 93 was treated with six concentrations of EMS to determine the optimal concentration for generating large numbers of mutants without excessive reduction in germination. Based on overall survival, plant development, and yield of treated seed, 40 mM EMS was determined to be an appropriate concentration for the generation of a mutant population suitable for TILLING. Higher concentrations of EMS resulted in overall survival rates of less than 10%, which are considered inadequate for efficient mutagenesis and population development. The generation of this mutagenesis population will allow for the elucidation of the genetics of agriculturally important traits in common bean.

Technical Abstract: In this study, genotype BAT 93 was treated with six EMS concentrations, including 0, 20, 30, 40, 50, and 60 mM, to determine the best concentration for the development of a mutagenesis population. The mutant population will be used for performing reverse genetics through TILLING (targeted induced local lesions in genomes) in common bean. Germination, plant height, and seed yield component data were collected. Large reductions in overall survival occurred with concentrations of 30 mM or higher EMS, while plant height and yield components were also increasingly affected by higher mutagen concentrations. Based on overall survival, plant development, and yield of treated seed, 40 mM EMS was determined to be an appropriate concentration for the generation of a mutant population suitable for TILLING. Higher concentrations of EMS resulted in overall survival rates of less than 10%, which were considered inadequate for efficient mutagenesis and population development.