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ARS Home » Plains Area » Grand Forks, North Dakota » Grand Forks Human Nutrition Research Center » Dietary Prevention of Obesity-related Disease Research » Research » Publications at this Location » Publication #219640
Title: High dietary intake of sodium selenite does not affect gene mutation frequency in rat colon and liver

Author
item Zeng, Huawei
item Uthus, Eric
item ROSS, SHARON
item DAVIS, CINDY

Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 2/4/2008
Publication Date: 4/15/2008
Citation: Zeng, H., Uthus, E.O., Ross, S.A., Davis, C.D. 2008. High dietary intake of sodium selenite does not affect gene mutation frequency in rat colon and liver. [abstract] Journal of Federation of American Societies for Experimental Biology. 22:146.7.

Interpretive Summary:

Technical Abstract: Gene mutations have been implicated in the etiology of cancer. In the present study, we utilized Big Blue transgenic rats to evaluate the in vivo mutation frequency of the ' cII gene in rats fed either a Se-deficient (0 µg Se/g diet) or selenium-supplemented diet (2 µg Se/g diet) (n=6 rats/diet) and injected with dimethylhydrazine (DMH) (25 mg/kg body weight, i.p.). There were no differences in body weight (252~264 g) between the Se-deficient and Se-supplemented rats, but liver glutathione peroxidase and thioredoxin reductase activities were significantly lower (p<0.0001) in Se-deficient rats (17.20 and 1.24 U/mg) compared to rats supplemented with Se (607.68 and 4.59 U/mg, respectively). Gene mutation frequency was significantly lower in liver (140~146 mutants per 106 ' cII gene sites) than in colon (579~604 mutants per 106 ' cII gene sites), and there were no differences in gene mutation frequency in DNA from colon mucosa or liver from rats fed the Se-deficient diet compared to those fed the Se-supplemented diet. While previous studies have shown that selenium is protective against DMH-induced preneoplastic colon cancer lesions, the current results suggest that it does not inhibit DMH-induced mutations in the ' cII gene.