Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 7/30/2006
Publication Date: 7/15/2007
Citation: Burri, B.J. Analysis of vitamin e by hplc. Book Chapter. 2007. The Encyclopediqa of Vitamin E (eds V.R. Preedy and R.R. Watson)pp.172-181.
Interpretive Summary: High performance liquid chromatography (HPLC) is the most common method for measuring vitamin E. This paper reviews the common HPLC methods for vitamin E. Moreover, it describes two well-established methods for vitamin E analysis in detail. The first method is a simple method that measures only the most common form of vitamin E. It can be used by scientists even if they have an old and inexpensive HPLC. The second method measures vitamin E and also vitamin A, beta-carotene, alpha-carotene, lutein, lycopene, zeaxanthin and beta-cryptoxanthin. This method requires a better HPLC. However, it measures a selection of nutrients that many nutritionists want to study.
Technical Abstract: HPLC (High-performance liquid chromatography) is the most comon technique for identifying and measuring vitamin E concentrations. A variety of good HPLC methods are available for vitamin E analysis. Reliable and sensitive methods have been developed using reversed-phased and normal-phase HPLC columns, isocratic and gradient elutions, and fluorescent, electrochemical and ultraviolet detectors. Satisfactory internal standards include tocol, tocopheryl acetate and retinyl acetate. Current methods development focuses on analysing vitamin E in difficult sample matrices, analysing vitamin E metabolites and most commonly on combining vitamin E analysis with the analysis of other micronutrients such as vitamin A and the carotenoids.