Submitted to: Master Brewers Association of the Americas Technical Quarterly
Publication Type: Review Article
Publication Acceptance Date: 7/25/2007
Publication Date: 9/13/2007
Citation: Schmitt, M., Budde, A.D. 2007. Use of fluorogenic proteinase assays to examine protein mobilization in barley varieties and across populations. Master Brewers Association of the Americas Technical Quarterly. 44(4):273-277.
Technical Abstract: Improved methods for efficient sample extraction and proteinase assay have simplified estimation of malt proteinase activity, making feasible examination of larger sample sets than was previously possible. The ability to characterize various proteinase activities across > 1000 lines enables use of comprehensive, structured populations of barley germplasm to better understand the interactions between proteinase activities, protein modification, and other contributors to desirable malt characteristics. Application of the assays to a snapshot of US barley lines highlights differences between cysteine and serine class endoproteinase functions during barley germination and suggests previously unsuspected linkages between protein and carbohydrate catabolism in the germinating seed.