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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #212467
Title: Detection of respiratory viruses and subtype identification of influenza viruses by GreeneChipResp oligonucleotide microarray

Author
item QUAN, P
item PALACIOS, G
item JABADO, O
item CONLAN, S
item HIRSCHBERG, D
item POZO, F
item JACK, P
item CISTERNA, D
item RENWICK, N
item HUI, J
item DRYSDALE A, A
item BAUMEISTER, E
item SAVY, V
item Lager, Kelly
item Richt, Juergen
item BOYLE, D
item WEBBY, R
item GARCIA-SASTRE, A
item CASAS, I
item PEREX-BRENA, P
item BRIESE, T
item LIPKIN, W

Submitted to: Journal of Clinical Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/21/2007
Publication Date: 8/1/2007
Citation: Quan, P.L., Palacios, G., Jabado, O.J., Conlan, S., Hirschberg, D.L., Pozo, F., Jack, P.J., Cisterna, D., Renwick, N., Hui, J., Drysdale A., Amos-Ritchie, R., Baumeister, E., Savy, V., Lager, K.M., Richt, J.A., Boyle, D.B., Garcia-Sastre, A., Casas, I., Perez-Brena, P., Briese, T., Lipkin, W.I. 2007. Detection of respiratory viruses and subtype identification of influenza A viruses by GreeneChipResp oligonucleotide microarray. Journal of Clinical Microbiology. 45(8):2359-2364.

Interpretive Summary: Influenza A virus infections are a significant causes of morbidity and economic burden for the pig industry. We have developed a fast and reliable detection system for influenza A viruses of pigs. All influenza viruses circulating in U.S. pigs can be easily identified using this detection system. In addition, this assay allows accurate identification of 21 different respiratory virus species from animals and humans. This information will be useful to livestock health officials and USDA action agencies such as USDA/APHIS.

Technical Abstract: Acute respiratory infections are a significant cause of morbidity, mortality and economic burden worldwide. Accurate, early differential diagnosis may alter individual clinical management as well as facilitate recognition of outbreaks that have implications for public health. Here we report the establishment and validation of a comprehensive and sensitive microarray system for detection and speciation of respiratory viruses in clinical materials. Implementation of a set of influenza enrichment primers facilitated subtyping of influenza A viruses through differential recognition of hemagglutinins 1 through 16 and neuraminidases 1 through 9. Twenty-one different respiratory virus species were accurately characterized including a recently identified novel genetic clade of rhinovirus.