Submitted to: Society for Leukocyte Biology Meetings Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/2/2007
Publication Date: 10/11/2007
Citation: Swaggerty, C.L., Chiang, H.I., Zhou, H., Li, X., Pevzner, I.Y., Kogut, M.H. 2007. Gene expression profiling of heterophils from Salmonella-resistant and-susceptible chickens using a 44K agilent microarray [abstract]. In: Proceedings of the Society for Leukocyte Biology, October 11-13, 2007, Cambridge, Massachusetts. p. 20.
Technical Abstract: We previously examined in vitro heterophil function and resistance/susceptibility to in vivo challenge with Salmonella enteritidis (SE) in broilers. All evaluations have shown a dichotomy of innate immune responsiveness with line A more responsive with increased ability to phagocytize, degranulate, produce an oxidative burst response, and mRNA expression of pro-inflammatory cytokines compared to line B. The in vitro studies translated to increased resistance (A) and susceptibility (B) in in vivo challenges with SE. Microarrays allow us to perform large-scale expression profiling to ascribe biological function and interactions between genes with available genomic sequences. Heterophils were isolated from line A and B chickens, stimulated with SE, and RNA isolated and analyzed with a chicken 44K Agilent microarray. A dual-color balanced design provided a direct comparison between SE-treated and control heterophils (A-SE vs. A-Con; B-SE vs. B-Con) and between lines (A-Con vs. B-Con; A-SE vs. B-SE). There were more (P<0.05) genes expressed in A-Con (4377) compared to B-Con (4333) heterophils. Of those, 71 and 69, respectively, were immune-related. Following exposure to SE, fewer (P<0.05) genes were expressed in A (3096) compared to B (3312). Even though fewer total genes were expressed in SE-treated line A heterophils, 67 were immune-related compared to 56 for line B. These results provide data to dissect the genes associated with resistance against SE in chickens.