|Porch, Timothy - Tim|
Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 5/23/2007
Publication Date: 5/26/2007
Citation: Porch, T.G., Lariguet, P., Blair, M., Broughton, W. 2007. EMS mutagenesis of BAT 93 for TILLING. Proceedings of Phaseomics V:16. May 23-26, 2007, Varenna, Italy.
Technical Abstract: TILLING (targeted induced local lesions in genomes) offers a unique opportunity for performing reverse genetics in common bean due to the absence of an effective transformation protocol and thus the inability to develop transposon-based or T DNA-based mutagenesis systems. TILLING is complementary to traditional plant breeding programs as it provides a source of characterized genes for gene-based marker development for use in molecular selection of important traits (e.g. for nutritional quality, yield, or disease resistance). EMS (ethyl methane sulfonate) induced mutagenesis creates numerous point mutations in the genome, with the mutagenesis efficiency based on the EMS concentration, the length of the treatment, and the ambient temperature. This study evaluated EMS concentrations of 20 to 60mM in order to confirm approriate levels of EMS for TILLING of the genotype BAT 93. Germination, plant survival, plant height, and seed yield were evaluated based on protocols developed previously (C. Pankhurst, P. Lariguet and W. Broughton, unpublished) using a 15 hour EMS treatment at 21°C. Development of the mutagenesis population is being carried out collaboratively at the University of Geneva in Geneva, Switzerland, at the International Center for Tropical Agriculture (CIAT) in Palmira, Colombia, and at USDA-ARS-TARS in Mayaguez, Puerto Rico.