Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: 2/15/2007
Publication Date: 7/20/2007
Citation: Njuguna, W., Bassil, N.V., Davis, T. 2007. Genetic Diversity of Fragaria iinumae and F. nipponica Based on Microsatellite Markers. HortScience. 42(4):915. Interpretive Summary: The United States Department of Agriculture (USDA) - Agricultural Research Service (ARS) - National Clonal Germplasm Repository (NCGR) in Corvallis, Oregon, preserves strawberries as plant collections and as seed. Seventeen different species from 37 countries are represented in the collection. Wild relatives of the domestic strawberry are conserved by maintaining them as seed lots or as individual plants from each geographical area, when possible. In 2004, two Asian diploid species were collected during an expedition in Hokkaido, Japan from 22 different locations. Previous studies have suggested that one of these diploids is an ancestor of the cultivated strawberry. The objectives of this study were to evaluate the diversity of these two Asian species using molecular techniques and to identify plants that represent the diversity in these two species. Conservation of strawberry and its wild relatives ensures their availability for cultivar improvement by breeders and further research by scientists.
Technical Abstract: The United States Department of Agriculture (USDA) - Agricultural Research Service (ARS) - National Clonal Germplasm Repository (NCGR) in Corvallis, Oregon, is a genebank that preserves strawberry genetic resources. The Fragaria L. collection consists of accessions from 17 species and 37 countries. In 2004, two Asian diploid species, F. iinumae Makino and F. nipponica Makino were collected during an expedition in Hokkaido, Japan. The accessions were collected as seed (extracted from ripe fruit) and runner plants from 22 locations throughout Hokkaido (11 locations for each species). F. iinumae, or its ancestor, may be a progenitor of the “B” genome for the cultivated octoploid strawberry, F. x ananassa Duschesne. The objectives of this study were to evaluate the diversity of these two Asian species using microsatellite or simple sequence repeat (SSR) markers, and to determine the minimum number of seedlings that represents the diversity found in the parent populations. Twenty-two out of 72 Fragaria derived SSRs amplified in these two species. We will describe the diversity of 81 F. iinumae and 63 F. nipponica accessions using the 22 SSR markers.