|Hotchkiss, Michael - Mike|
Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/15/2007
Publication Date: 7/28/2007
Citation: Livingston, S., Chen, J., Chang, C.J., Hopkins, D., Hotchkiss, M.W., Reilly, C.C., Civerolo, E.L. 2007. Genotypic and phenotypic characterization of Xylella fastidiosa strains isolated from California, Georgia and Florida. [abstract]. Phytopathology. 97:S67.
Technical Abstract: Xylella fastidiosa is an important plant pathogen causing Pierce’s Disease in grapes and in many other economically important crops. Because of its nutritional fastidiousness, characterization of this bacterium remains highly challenging, thereby hindering future scientific research and disease control. We isolated X. fastidiosa strains from over ten different plant hosts from California, Georgia, and Florida. These strains were characterized genotypically at the highly conserved 16S rDNA locus and the highly variable pspB locus. We also characterized the X. fastidiosa strains phenotypically in colony morphology and growth in different culture media. Three single nucleotide polymorphisms (SNPs) can un-ambiguously divide these strains into two groups: A- and G-genotypes. The two groupings are supported by the phenotypic analyses. On the other hand, tandem repeat numbers and patterns in the pspB locus could subdivide each 16S rDNA genotypes into different groups according to host origins.