Submitted to: Plant Pathology
Publication Type: Peer reviewed journal
Publication Acceptance Date: 2/7/2007
Publication Date: 5/29/2007
Citation: Gillaspie Jr, A.G. 2007. Attempts to Improve the Method of Screening Cowpea Germplasm for Resistance to Cucumber Mosaic Virus and Blackeye Cowpea Mosaic Virus. Plant Pathology Journal.6(2):202-205. Interpretive Summary: Results show that the presence or absence of visual virus symptoms from plots can be used to select cowpea lines for further testing for Blackeye Cowpea Mosaic Virus (BICMV) resistance in greenhouse and field tests with inoculated spreader plants, but not for cucumber mosaic virus screening. This could save in time and funds for screening to control cowpea stunt, the most severe disease of cowpeas in the U.S. Besides the method, eleven new lines were identified with Blackeye Cowpea Mosaic Virus resistance potential.
Technical Abstract: Use of visual symptom screening for cowpea plants in field plots improved screening for Blackeye Cowpea Mosaic Virus (BICMV)-resistance. However, the method failed to improve the speed or accuracy of screening for Cucumber Mosaic Virus (CMV)-resistance. Plants that displayed few visual virus symptoms were selected for screening by a previously published method. This method involved screening by mechanical virus inoculation in the greenhouse. Plants having a low infection percentage in the greenhouse as judged by direct-antigen coating enzyme-linked immunosorbent assay (DAC-ELISA) were then screened in the field by randomized virus spread tests from inoculated spreader rows. Infection rates in these tests were also determined by DAC-ELISA. The test resulted in the detection of eleven newly-discovered sources of resistance to BICM, but no significant new sources of CMV-resistance were found.