Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: 9/26/2006
Publication Date: 1/13/2007
Citation: Wang, M.L., Morris, J.B., Barkley, N.L., Dean, R., Jenkins, T., Pederson, G.A. 2007. Genetic Diversity of Lablab (L. purpureus) Germplasm Assessed by SSR Markers. Plant & Animal Genome XV.p.112
Technical Abstract: The genetic diversity of the USDA Lablab purpureus germplasm collection is unknown and was assessed by using polymorphic simple sequence repeat (SSR) markers derived from Medicago, soybean and cowpea. Phylogenetic analysis partitioned 47 representative accessions into two main clades (wild clade producing small seeds and cultivated clade producing large seeds). Accessions in the wild clade could be distinguished from each other, whereas accessions in the cultivated clade cannot be easily resolved into clear goups. The wild clade is clearly diverged from the cultivated clade. The USDA cultivated subsp. purpureus collection contained moderate (limited) genetic diversity and needs to be expanded. Some lablab amplicons were sequenced. The sequence similarity to Medicago truncatula was variable from amplicon to amplicon; however, sequence analysis of lablab amplicons from the same SSR marker showed that the sequence content of these amplicons was almost identical, except for some single nucleotide polymorphisms (SNPs). Therefore, amplicons with different sizes from transferred SSRs can be used as DNA markers to distinguish germplasm accessions at the DNA fragment level. Furthermore, our genetic classification result was confirmed by morphological examination and sequence analysis.