Submitted to: Keystone Symposia
Publication Type: Abstract Only
Publication Acceptance Date: 10/6/2006
Publication Date: 1/5/2007
Citation: Swaggerty, C.L., Pevzner, I.Y., Kogut, M.H. 2007. Differential JAK response in polymorphonuclear cells from a non-mammalian species following exposure to Salmonella enteritidis [abstract]. In: Proceedings of Keystone Symposia on Molecular and Cellular Biology - Jaks, Stats and Immunity, January 5-10, 2007, Steamboat Springs, Colorado. p. 66.
Technical Abstract: The Janus family of tyrosine kinases (JAKs) are non-receptor tyrosine kinases which associate with cytokine receptors and are activated by ligand binding and subsequently auto-phosphorylate. JAK1 is involved in numerous signal transduction pathways, specifically interleukin (IL)-2, IL-6 and interferon (IFN) alpha, beta, and gamma receptors. We have extensively characterized the innate immune response of two parental lines (A and B) of chickens and shown that heterophils from B chickens have high mRNA expression levels of IFN-alpha following agonist stimulation while expression levels in A were unchanged from controls. Knowing there is a differential IFN-alpha response between the lines, we wanted to quantitate the levels of JAK in heterophils from A and B chickens. Heterophils were isolated from 4-day-old chickens, stimulated with a phagocytic agonist, Salmonella enteritidis (SE), and JAK levels quantitated. Total JAK levels in control heterophils were higher in B compared to A. Further, the differences between A and B were maintained following SE-phagocytosis. These data provide initial information that differences between the IFN-' mRNA expression of line A and B chickens is likely due, in part, to a differential signaling pathway(s).