Submitted to: Food Safety Consortium Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 8/28/2006
Publication Date: 10/3/2006
Citation: Huff, G.R., Huff, W.E. 2006. Food Safety Consortium Progress Report - Listeria Monocytogenes in Turkeys. In: Proceedings of Food Safety Consortium, October 1-3, 2006, Fayetteville, Arkansas. 2006 CDROM.
Technical Abstract: Work this past year has focused on the development of both a cold stress model and an Escherichia coli co-infection model of Listeriosis in turkey poults that are challenged by environmental exposure to Listeria monocytogenes Scott A (Lm). A Taqman real time PCR assay is currently being optimized for detection and quantitation of Lm from the joints and tissues of turkeys challenged in these experimental infections. The intracellular nature of Lm colonization is being addressed by utilizing an avian macrophage cell line to encourage intracellular growth and amplify joint isolates prior to molecular detection. Detection of Lm Scott A from the joints of environmentally challenged turkeys supports the hypothesis that processing plant contamination can originate from opportunisitic infections in the synovial tissues of fast-growing male turkeys and can be spread through the plant when turkeys are cut-up for further processing.