Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/6/2006
Publication Date: 8/6/2006
Citation: Nol, P., Waters, W.R., Palmer, M.V., Aldwell, F.E., Buddle, B.M., Rhyan, J.C., Dunbar, M., Salman, M.D. 2006. Efficacy of Oral and Parenteral Bacille Calmette-Guerin (BCG Danish Strain 1331) in Protecting White-tailed Deer (Odecoileus Virginianus) against Bovine Tuberculosis [abstract]. p. 25.
Technical Abstract: Wildlife Disease Association Annual Conference, August 6-10, 2006 Terry Amundson Student Presentation Award Oral Presentation EFFICACY OF ORAL AND PARENTERAL BACILLE CALMETTE-GUERIN (BCG DANISH STRAIN 1331) IN PROTECTING WHITE-TAILED DEER (ODECOILEUS VIRGINIANUS) AGAINST BOVINE TUBERCULOSIS Pauline Nol, USDA/APHIS National Wildlife Research Center, Fort Collins, CO, 80521; W. Ray Waters, USDA/ARS National Animal Disease Center, Ames, IA, 50010; Mitchell V. Palmer, USDA/ARS National Animal Disease Center, Ames, IA, 50010; Frank E. Aldwell, University of Otago, Dunedin, NZ; Bryce M. Buddle, AgResearch, Wallaceville Animal Research Centre, Upper Hutt, NZ; Jack C. Rhyan, USDA/APHIS National Wildlife Research Center, Fort Collins, CO, Mike Dunbar, USDA/APHIS National Wildlife Research Center, Fort Collins, CO 80521; Mo D. Salman, Department of Clinical Sciences, Colorado State University, Fort Collins, CO, 80523 Bovine tuberculosis poses a serious continual threat to the health and economic well-being of both livestock and humans worldwide. Free-ranging white-tailed deer (Odecoileus virginianus) in the state of Michigan (USA) have been shown to be endemically infected with Mycobacterium bovis, and serve as a potential reservoir of infection to livestock, other wildlife species, and humans, representing a significant obstacle to that state’s disease eradication efforts. An effective vaccination program, involving a mucosal vaccine that can be distributed in the field, would be essential for disease management on a herd-wide level. We investigated the efficacy of oral and parenteral bacille Calmette-Guerin (BCG) in its ability to protect white-tailed deer against disease caused by M. bovis infection. Thirty white-tailed deer were divided into four groups. One group was vaccinated with 10**6 colony forming units (cfu) BCG (Danish strain 1331) subcutaneously, one group received 10**9 cfu BCG in culture directly to the oropharynx, one group received 10**9 cfu BCG via a lipid-formulated oral bait, and the last group received a placebo directly to the oropharynx. Blood samples were taken throughout the study in order to investigate levels of cellular and humoral responses in this species to BCG vaccination and M. bovis challenge after vaccination. Four months post-challenge, the animals were examined for lesions. Results indicate that both oral forms of BCG offered significant protection against M. bovis challenge as compared to placebo.