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ARS Home » Southeast Area » Miami, Florida » Subtropical Horticulture Research » Research » Publications at this Location » Publication #199158
Title: THE DEVELOPMENT OF MICROSATELLITE MARKERS FOR PERSEA AMERICANA (AVOCADO).

Author
item Borrone, James
item VIOLI, HELEN - UNIV OF FL, IFAS
item PLOETZ, RANDY - UNIV OF FL, IFAS
item Schnell Ii, Raymond

Submitted to: Florida State Horticulture Society and Citrus Industry
Publication Type: Proceedings
Publication Acceptance Date: 10/25/2006
Publication Date: 6/15/2007
Citation: Borrone, J.W., Violi, H.A., Ploetz, R., Schnell II, R.J. 2007. The development of microsatellite markers for persea americana (avocado). Florida State Horticulture Society. 119:38-40.

Interpretive Summary: Sequence information in publically available databases was investigated to facilitate developing additional genetic markers for avocado. To date, 6183 sequences were examined, and 100 were selected for development as markers. This is the first step in developing genetic markers for avocado to select for individual trees displaying increased resistance to root rot.

Technical Abstract: Because of their relative abundance, distribution across the genome, hypervariability and co-dominance, microsatellites, or simple sequence repeats are considered to be cost effective and efficient molecular genetic markers for parentage analysis, linkage mapping, association studies, and genetic fingerprinting of cultivars. Currently, there is a small set of microsatellite markers available for Persea americana. We are expanding the number of available microsatellite molecular markers by screening pre-existing avocado expressed sequeance tag (EST)libraries. To date over 6183 sequences have been screened and 100 have been selected for marker development based upon the size, location, and type of microsatellite identified. The development of an additional 100 SSR markers, along with the currently available SSR markers, should provide suitable coverage of the avocado genome for assiciation studies and linkage mapping. These are the first steps in devising a marker assisted selection program for avocado.