Submitted to: Avian Immunology Research Group Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/20/2006
Publication Date: 10/21/2006
Citation: He, H., Genovese, K.J., Kogut, M.H. 2006. Nitric oxide synthesis and cytokine gene expression in chicken monocytes stimulated with CpG-ODN and double-stranded RNA [abstract]. In: Proceedings of 09th Avian Immunology Research Group Meeting, October 21-24, 2006, Paris, France. p. 29. Interpretive Summary:
Technical Abstract: Toll-like receptors (TLRs) recognize microbial components and initiate innate immune responses that control microbial infections. We have investigated the innate immune response of chicken monocytes to bacterial CpG-motif containing oligodeoxydinucleotide, CpG-ODN, and the analog of viral double-stranded RNA, poly I:C, by measuring the induction of NO synthesis and cytokine gene expression. Our results show ligands poly I:C and CpG-ODN of the TLR3 and TLR9, respectively, synergized the induction of NO in chicken monocytes. When stimulated separately, CpG-ODN induced NO production in the chicken monocytes; whereas, poly I:C stimulated very little NO production. In combination, CpG-ODN and poly I:C synergize the effect on NO synthesis, which results in significantly higher level of NO production in chicken monocytes. This synergistic effect of CpG ODN-ODN and poly I:C was also observed on the expression of IL-1beta and IL-12 mRNA. In addition, IL-18 gene expression was differential induced by poly I:C stimulation of monocytes, but not by CpG-ODN stimulation. Those results demonstrate differential roles for TLR3 and TLR9 in signaling immune responses to bacterial and viral infections in chicken monocytes as well as a synergistic interaction between the two signaling pathways.