Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/10/2006
Publication Date: 9/10/2006
Citation: Yakes, B.J., Lipert, R.J., Bannantine, J.P., Porter, M.D. 2006. Novel detection method for Mycobacterium avium subsp. paratuberculosis using surface-enhanced Raman scattering based immunoassays [abstract]. 232nd American Chemical Society National Meeting.
Technical Abstract: Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of Johne's disease in cattle, imparts a loss of over $200 million to the U.S. dairy industry each year. One of the major obstacles in controlling the spread of this highly prevalent disease is the facile detection of the bacteria at low, subclinical levels. This paper introduces innovations that overcome both obstacles by using a sandwich immune assay, which is based on immunogold nanoparticle labels and surface-enhanced Raman scattering. By using monoclonal antibodies that are highly specific for a MAP surface protein, this labeling strategy yields large Raman signals that translate to a detection limit of ~500 bacteria/mL. This presentation will detail this assay, present evidence for shedding of the surface protein, and demonstrate its performance in complex sample matrices.