Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/20/2006
Publication Date: 3/9/2006
Citation: Figueroa, D., Amarillo, I.E., Ring, B., Strobel, C., Lawrence, C.J., Bass, H.W. 2006. Constructing a cytogenetic map of maize core bin markers in oat addition lines using sorghum BACs as FISH probes [abstract]. 48th Annual Maize Genetics Conference Program and Abstracts. p. 71. Interpretive Summary:
Technical Abstract: We are developing a pachytene cytogenetic FISH map of the maize genome using sorghum BACs corresponding to the 90 maize Core Bin Marker (CBM) loci. These loci were chosen because they are uniformly distributed and they delineate the genetic bins derived from the UMC98 (Genetic 2005) maize linkage maps. We are using the single-locus cytogenetic FISH mapping system previously described by Koumbaris and Bass (2003, Plant J. 35:647). Maize marker-selected sorghum BACs are used as FISH probes on maize pachytene chromosome spreads from alien addition lines of oat. Progress on maize 9 is described by Amarillo et al. (accompanying poster). The project presented here is specifically focused on mapping chromosomes 1, 3, 4, 5, 6, and 8. The procedure for selecting a sorghum BAC is illustrated here using the RFLP probe umc161 (CBM 1.11, GenBank Acc. AY771212). In addition, we have been obtaining full-length insert sequences for all of the CBM probes as summarized online (http://www.plantgdb.org/prj/RFLP_FLIS/). The project is described at cytomaize.org with mapping, image, and RFLP sequence data being released through NCBI, MaizeGDB.org, and plantgdb.org. This project will provide insights into the structure of the maize genome, create new technologies and reagents for chromosome research, and assist in genome assembly and integrating the physical, genetic, and cytological maps of maize with those of sorghum.