|Swaggerty, Christina - Christi|
|He, Louis - Haiqi|
|Genovese, Kenneth - Ken|
|Kogut, Michael - Mike|
Submitted to: Congress International Society Develop Comparative Immunology
Publication Type: Abstract Only
Publication Acceptance Date: 2/21/2006
Publication Date: 7/1/2006
Citation: Swaggerty, C.L., He, H., Genovese, K.J., Kaiser, P., Pevzner, I.Y., Kogut, M.H. 2006. The feathering gene influences degranulation and oxidative burst but not cytokine/chemokine mRNA expression or Salmonella enteritidis organ invasion in broilers [abstract]. In: Proceedings of 10th International Congress of Developmental and Comparative Immunology, July 1-6, 2006, Charleston, South Carolina. p. 236.
Technical Abstract: In the past, we showed differences in in vitro heterophil function between parental broilers (Line A>B and Line X>Y) and F1 reciprocal crosses (Line C<D) and determined the presence of the feathering gene influences heterophil function. Specifically, the fast feathering gene (k) may enhance heterophil functional efficiency while the slow feathering gene (K) reduces efficiency. Based on earlier studies, we want to identify the role of the feathering gene in innate immune response of broilers. Heterophils were isolated from two-day-old Line C and D chickens separated into males (CM and DM) and females (CF and DF). With respect to the feathering gene, CM=DM (K/k+) whereas CF and DF are K/- and k+/-, respectively. Therefore, if in vitro heterophil functional is associated with the feathering gene; heterophils from DF would be functionally efficient compared to heterophils from CF while CM and DM would be equal. Heterophil functions of degranulation and oxidative burst were measured and DF were more (p<0.05) efficient than CF, while the males were comparable indicating the feathering gene is linked to these two functions. Additionally, interleukin (IL)-6 and CXCLi2 (formerly IL-8) mRNA expression were quantitated by real-time quantitative RT-PCR and there were no differences between the groups. Lastly, two-day-old chickens were administered SE and liver/spleen invasion evaluated and the differences are not attributed to the feathering gene. In conclusion, the data indicate the biochemical killing mechanisms of degranulation and oxidative burst are under the control of the feathering gene. However, cytokine/chemokine mRNA expression and SE organ invasion are not influenced by the feathering gene.