Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/23/2006
Publication Date: 7/15/2006
Citation: Mousel, M.R., Nonneman, D.J., Rohrer, G.A. 2006. Rearranged gene order between pig and human in a quantitative trait loci region on SSC3. Animal Genetics. 37:403-406
Interpretive Summary: A region of pig chromosome 3 has been associated with a potentially large effect on litter size in pigs by increasing the number of eggs ovulated. To enable targeted marker development and produce markers that could be used for commercial pig production, a detailed comparative map between the pig and human genome needs to be created. Therefore, a study to map genes expected to be located on pig chromosome 3 was initiated. The results of this study placed 52 genes on the physical map of pig chromosome 3 that are located on human chromosomes 2, 7 and 16. The order of the genes in the pig genome indicate numerous rearrangements have occurred as pigs and humans have evolved. These data refine the comparative map between human and pig and will facilitate future studies in this economically important region of the pig genome.
Technical Abstract: A QTL on chromosome 3, peak at 36 cM, has been identified in a Meishan-White Composite resource population that is significantly correlated with ovulation rate (OR) and has a large effect (2.2 corpora lutea). In an effort to identify the gene(s) responsible, an increase in the density of the genes on the IMpRH map of SSC3 and comparative analysis of gene order, was conducted. This study placed 52 known genes and ESTs, two BAC end sequences and one microsatellite (SB42) on a framework map that was able to fill gaps on previous RH maps. Data were analyzed for two-point and multipoint linkage with the IMpRH mapping tool and submitted to the IMpRH database (http://imprh.toulouse.inra.fr/). Carthagene (http://www.inra.fr/bia/T/CarthaGene/) was used to estimate multipoint marker distance and order using all public markers on SSC3 in the IMpRH database and those developed in this study. Comparative analysis of human and porcine maps identified conservation of gene order of the q arm and the telomeric region of the p arm for SSC3. Three separate human chromosomes were represented and 7 breakpoints were determined in the studied region of SSC3. Gene order was confirmed for closely linked loci by using the high resolution IMNpRH2 panel. These data refine the human-porcine comparative map and placed additional genes on SSC3 within an OR QTL.