Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/1/2005
Publication Date: 10/23/2005
Citation: Clements, M.J., Williams, W.P., Moore, S.P., Krakowsky, M.D., Guo, B., White, D.G., Xu, W., Isakeit, T., Brooks, T.D., Windham, G.L., Abbas, H.K., Perkins, J., Gorman, D., Raab, Q., Arnold, K., Smith, D., Betran, J. 2005. Southern east regional aflatoxin test (serat) [abstract]. In: Proceedings of the 2005 Multicrop Aflatoxin/Fumonisin Elimination & Fungal Genomics Workshop, October 23-27, 2005, Raleigh, North Carolina. p. 81.
Technical Abstract: A testing network has been established to identify corn germplasm that contributes resistance to aflatoxin accumulation in grain to hybrid performance across multiple environments. Southern East Regional Aflatoxin Test (SERAT) is a multi-organization, multi-state evaluation of the most promising germplasm from several research groups. Research groups provide seed of a few hybrids and a testing location. In addition to aflatoxin concentration in grain, traits examined include grain yield, maturity, lodging, grain moisture, test weight, etc. In 2004, SERAT trials were evaluated at six locations: Alexandria, LA; Tifton, GA; Starkville, MS; Urbana, IL; Halfway, TX; and Weslaco, TX. Primary ears on all plants were inoculated via a pinboard method (Urbana), via injection of inoculum through husk leaves into the side of the ear (Starkville), or via injection of inoculum down the silk channel (all other locations). Significant genotype x environment interaction (GEI) was observed for aflatoxin and grain yield. Average aflatoxin was 730, 54, 392, 182, 21, and 652 ng g-1 at Alexandria, Tifton, Starkville, Halfway, Urbana and Weslaco, respectively. Principal component analysis supported the observation that aflatoxin concentration among genotypes is affected significantly by environment. In 2005, SERAT trials were evaluated at 9 locations: Alexandria, LA; Tifton, GA; Starkville, MS; Urbana, IL; Halfway, TX; Weslaco, TX; Ganado, TX; Mistic, GA; and Claxton, GA. Primary ears on all plants were inoculated using methodology described for 2004. Additionally, ears at Ganado were inoculated by injection of inoculum down the silk channel, and ears at Mistic and Claxton were naturally infected. As in 2004, highly significant GEI was observed for aflatoxin concentration and grain yield. Average aflatoxin concentration was 275, 587, 118, and 134 ng g-1 at Alexandria, Tifton, Ganado, and Weslaco, respectively. Aflatoxin concentrations from the remaining locations are being quantified. Average grain yield was 88, 166, 117, 131, 129, 118, and 116 bu A-1 at Weslaco, Mistic and Claxton combined, Ganado, Tifton, Starkville, Halfway, and Alexandria, respectively. A number of hybrids have potential as sources of favorable agronomics, while others have potential as sources of resistance to aflatoxin accumulation in grain. This suggests possibilities of combining positive traits by crossing germplasm from different programs. SERAT has promoted collaboration among research groups across multiple states and organizations, and added to the knowledge of genotype x environment interaction related to aflatoxin accumulation in grain.