|Porch, Timothy - Tim|
Submitted to: International Conference on Legume Genomics and Genetics
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2004
Publication Date: 6/7/2004
Citation: Lariguet, P., Pankhurst, C.E., Porch, T.G., Silue S., Boukli, N.M., Ishitani, M., Rao, I.M., Beebe, S., Triplett, E.W. Baudoin, J.P., Blair, M.W., Broughton, W.J. 2004. Tilling beans for changes in nodule and seed development as well as tolerance to aluminum [abstract]. International Conference on Legume Genomics and Genetics. Interpretive Summary:
Technical Abstract: Beans are the most important grain legumes for direct human consumption in the world, particularly for poor people in Latin America and Africa, and for this reason are the model food-legume of choice (Broughton et al., 2003). Rapid improvement of their agronomic and nutritional characteristics is dependent on developing a genetic platform that includes mutagenesis and screening protocols that will permit plant scientists all over the world to conduct both forward and reverse genetics. Thus an ethyl methane sulphonate (EMS)-induced mutant collection of common beans is being created at the Université de Genève, the USDA in Puerto Rico, and CIAT in Cali, Columbia. Seeds of the EMS collection will be freely made available to researchers for phenotypic screening of traits of interest. This collection now comprises 1,008 M2 lines and is being used to isolate mutants deficient in nodulation, seed development and resistance to soil aluminium. Of the 340 M2 lines tested, 29 plants with altered nodulation patterns have been identified. In another screen, seeds of two plants (out of 62 tested) failed to develop normally while seeds of another two mutants aborted within 15 to 25 days after anthesis. In addition to screening for mutants, seed development will be followed using proteomic approaches. TILLING (Targeted Induced Local Lesions IN Genomes) protocols are being developed to isolate these lesions, and large-scale random-sequencing of the P. vulgaris genome has been initiated.