Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/1/2005
Publication Date: 10/23/2005
Citation: Kelley, R.Y., Boykin, D.L., Hawkins, L.K., Williams, W.P. 2005. Differential gene expression levels of Aspergillus flavus resistance in two inbred maize lines [abstract]. In: Procedings of the 2005 Multicrop Aflatoxin/Fumonisin Elimination & Fungal Genomics Workshop, October 23-27, 2005, Raleigh, North Carolina. p. 44.
Technical Abstract: The objective of this experiment was to identify differentially expressed genes for Aspergillus flavus resistance in the Va35 (susceptible) and Mp313E (resistant) maize (Zea mays L.) lines using cDNA microarray analysis. Primary ears from plants in treated plots were inoculated with isolate NRRL 3357 of A. flavus 14 days after pollination and were harvested two days after inoculation. Uninoculated ears were harvested 16 days after pollination and used as a control. cDNA from the inoculated and uninoculated ears was labeled with Cy3 and Cy5 fluorescent dyes. Both samples were hybridized to the unigene 1.01.05 maize chip containing 5,065 EST contigs from EST libraries derived from immature leaf, endosperm, immature ear and the root. Out of the 5065 ESTs analyzed, 123 or 2.4% of the total ESTs analyzed were significantly up-regulated for the susceptible inbred line Va35 and 95 or 1.8% of the total ESTs analyzed were significantly up-regulated for the resistant inbred line Mp313E, and 16 or 0.3% of the total ESTs were up-regulated for both the susceptible and resistant inbred lines. The expressed ESTs include genes with known functions such as stress response, metabolism, protein synthesis, cellular communication and signal transduction, transcription and RNA processing and photosynthesis-associated genes. Up-regulated ESTs also included genes with unknown functions, demonstrating the usefulness of microarray as a gene discovery tool. Results presented here provide an overview of Va35 and Mp313E functional genes that are expressed two days after inoculation with A. flavus.