Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/21/2005
Publication Date: 10/21/2005
Citation: Barkley, N.L., Newman, M.L. aka Harrison Dunn, M.L., Wang, M.L., Hotchkiss, M.W., Pederson, G.A. 2005. Do not be bamboozled by bamboo: a molecular study of the usda temperate bamboo collection. In: American Bamboo Society National Meeting Abstract.
Technical Abstract: The USDA bamboo germplasm collection began in 1919 in Savannah, GA and was later relocated to the USDA Southeastern Fruit and Tree Nut Research Laboratory in Byron, GA. The purpose of this collection is to provide a diverse set of bamboo species for use by researchers and commercial businesses. Molecular marker work was performed to investigate the genetic variability of the bamboo collection. EST-SSR markers derived from major cereal crops were utilized to assess their transferability to bamboo. The markers that were determined to be polymorphic from screening a set of bamboo accessions were selected to assess genetic diversity of the USDA temperate bamboo collection, consisting of 92 accessions classified in 11 separate genera and 44 species. A total of 211 bands were detected with a mean number alleles per locus of 8.440. Phylogenetic relationships were determined by calculating genetic distances between all pair wise combinations (UPGMA) and assessing differences in character data (parsimony). These dendrograms clustered the accessions into two main clades which corresponded to accessions characterized as either clumping (sympodial) or running (monopodial) bamboos. The majority of the accessions clustered according to their taxonomic classification. These markers were also beneficial in identifying contaminated and misidentified plots. Overall, these transferred markers were informative in differentiating the various bamboo accessions and determining interspecific and intraspecific variation.