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United States Department of Agriculture

Agricultural Research Service

Title: DETECTING CMI RESPONSES TO MYCOBACTERIUM AVIUM SSP. PARATUBERCULOSIS INFECTION IN CALVES BORN TO NATURALLY INFECTED DAMS)

Author
item Robbe Austerman, Suelee
item Young, Sarah
item Oconnor, Annette
item Palmer, Mitchell
item Stabel, Judith

Submitted to: International Colloquium on Paratuberculosis
Publication Type: Abstract Only
Publication Acceptance Date: 5/11/2005
Publication Date: 8/14/2005
Citation: Robbe Austerman, S., Young, S., Oconnor, A., Palmer, M.V., Stabel, J.R. 2005. Detecting CMI responses to Mycobacterium avium ssp. paratuberculosis Infection in Calves born to Naturally Infected Dams [abstract]. International Colloquium on Paratuberculosis. p. 570.

Interpretive Summary: Our objectives were to evaluate the best age to use the skin test and INF-gamma ELISA for Johne’s disease in calves. Seventeen calves born from naturally infected Johne’s cows were used. At 4-6 months, 6-8 months and 12-13 months each calf was with the skin test and the INF-gamma ELISA. Calves were necropsied at 12-13 months of age and tissues were cultured. Ten of 17 calves were positive for Johne’s disease and 7 were negative. Of the ten calves the skin test identified 5 at 4-6 months of age, 4 at 6-8 months and 7 at 12-13 months. Of the ten Johne’s positive calves the INF-gamma ELISA identified 2 calves at 4-6 months of age, 4 at 6-8 months and 10 at 12-13 months. Of the seven Johne’s negative calves, all tested negative using the skin test except three animals at 12-13 months. The INF-gamma ELISA identified one calf as positive at 4-6 months of age, 6 as positive at 6-8 months and again at 12-13 months. In Johne’s positive calves the skin test and INF-gamma ELISA appeared best able to identify calves with Johne’s disease at 12-13 months.

Technical Abstract: Our objectives were to evaluate the age a CMI response could be detected in calves and the accuracy and reliability of the skin test and INF-gamma ELISA in MAP infected and non-infected calves. Seventeen calves born from naturally infected Johne’s cows were used. At 4-6 months, 6-8 months and 12-13 months each calf was tested for the presence of CMI response using the skin test and the INF-gamma ELISA. Calves were necropsied at 12-13 months of age and tissues were cultured. Ten of 17 calves were tissue culture positive for MAP and 7 negative. Of the ten MAP culture positive calves the skin test identified 5 at 4-6 months of age, 4 at 6-8 months and 7 at 12-13 months. Of the ten MAP culture positive calves the INF-gamma ELISA identified 2 calves at 4-6 months of age, 4 at 6-8 months and 10 at 12-13 months. Of the seven MAP culture negative calves, all tested negative using the skin test except three animals at 12-13 months. The INF-gamma ELISA identified one calf as having CMI response to MAP at 4-6 months of age, 6 as positive at 6-8 months and again at 12-13 months. In tissue culture positive calves the skin test and INF-gamma ELISA appears best able to identify a CMI response at 12-13 months. Even though the INF-gamma ELISA identified 6 of the 7 non-infected calves as positive, it may be the standard positive cutoff of 0.100 + control plasma is not adequate in young calves.

Last Modified: 05/26/2017
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