Submitted to: Safepork
Publication Type: Proceedings
Publication Acceptance Date: 7/7/2005
Publication Date: 9/6/2005
Citation: Bearson, S.M., Uthe, J.J., Royaee, A.R., Lunney, J.K., Zhao, S., Kim, J., Tuggle, C.K. 2005. The transcriptional response to Salmonella infection in swine. Safepork 2005. p. 68-71.
Technical Abstract: The porcine response to infection with Salmonella is the result of differential expression of host-specific genes. To characterize these alterations in gene expression, functional genomic analyses were performed on swine tissues following experimental inoculation of the pigs with Salmonella enterica serovars Choleraesuis and Typhimurium. Suppression subtractive hybridization and quantitative real-time RT-PCR revealed that the transcriptional profiles of the porcine response to the swine-adapted strain (Choleraesuis) and the non-host-adapted strain (Typhimurium) exhibit unique differences. Our research demonstrates not only a difference in gene expression in animals infected with two different strains of Salmonella (host-specificity), but also alterations in the transcriptome over a time course of infection from the acute state (48 hr) to the chronic or carrier stage (21 days).