Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/31/2004
Publication Date: 10/31/2004
Citation: Wang, M.L., Chen, Z., Barkley, N.L., Newman, M.L. aka Harrison Dunn, M.L., Kim, W., Raymer, P., Pederson, G.A. 2004. Development of a full set of molecular genetic markers for assessment of germplasm genetic diversity and protection of cultivar patent right of turfgrass. In: ASA-CSSA-SSSA Annual Meeting Abstracts, Madison, WI. CDROM.
Technical Abstract: One hundred and thirty SSR markers from wheat, maize, and sorghum were screened for the transferability to Paspalum. The transfer rate was 67.5%, 49.0%, and 66.8% respectively. This would be a very efficient approach for DNA marker development for species which are not well studied molecularly. The polymorphism level for transferred SSR markers was 51.5% within species (Paspalum vaginatum) and 87.1% among Paspalum species. The high level of polymorphism is directly related to the high degree of heterozygosity maintained by its way of reproduction, i.e., self-incompatability. Forty transferred polymorphic SSR markers were selected and used for characterization and evaluation of seventy-three Paspalum accessions. In total, 209 polymorphic bands were detected from these 40 SSR markers, with an average of five polymorphic bands per marker. The Paspalum accessions were genetically clustered into three major groups. Two very similar dendrograms can ben generated from either 109 or 209 polymorphic bands. This led us to determine that 20 of the transferred SSR markers were sufficient for genetically differentiating and clustering the investigated germplasm accessions. The number of SSR markers required for germplasm characterization and evaluation is discussed. This is the first report of the transfer of SSR markers from major field crops to newly-emerged environmental turfgrasses.