Submitted to: International Organization of Citrus Virologists Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/7/2004
Publication Date: 11/30/2004
Citation: Palmieri, M., Donis, I., Salazar, A.L., Cruz, N., Paniagua, A., Brlansky, A., Guerra-Moreno, A.S., Manjunath, K.L., Lee, R.F. 2004. A method to survey for citrus pathogens when biological indicator plants are not available. International Organization of Citrus Virologists Abstracts, Page 105. Interpretive Summary:
Technical Abstract: Technical Abstract: The use of biological indicator plants for indexing for graft transmissible pathogens of citrus is absolutely essential. However in Guatemala there was an urgent need to conduct a survey to identify what citrus pathogens were present, and no indicator plants were available. We describe a protocol based on total nucleic acid extractions made from field trees which was used to test 86 samples collected from Guatemala for the presence of tristeza, psorosis, exocortis, cachexia, group III viroids, leprosis, and Xylella fastidiosa. Exocortis, cachexia, and group III viroids, tristeza virus, and leprosis were found. The RT-PCR assays for psorosis were negative although symptoms in the field were similar to those expected for psorosis. Biological tests are now being performed to verify these results.