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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #176080


item Jenderek, Maria

Submitted to: International Plant Propagators Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 12/4/2000
Publication Date: 3/5/2001
Citation: Jenderek, M.M., Olney, R. 2001. Hibiscus syriacus plant regeneration from callus. International Plant Propagators Proceedings. V. 50. P. 565-568.

Interpretive Summary: Eastern hibiscus is difficult to propagate vegetatively because of the presence of pathogens existing in the plants, and a limited ability to proliferate shoots. The study investigated if growing plants from callus tissue derived from young seedlings without the pathogens may be an effective way to propagate the ornamental shrub. Fragments of three different seedling parts (root, hypocotyl and cotyledon) were grown in sterile conditions on artificial medium with different types of growth regulators and at different concentrations. The highest number of shoots was observed on cultures originated from cotyledon and shoot fragments. The most leaves were observed on callus initiated from root fragments. The plants grown from callus were cultivated for 30 days in trays and then transplanted to a greenhouse. One and half months later, 100% of the plants developed additional leaves and elongated shoots. NAA and BA callus induction medium and BA supplemented regeneration medium were the most suitable for micropropagation of the eastern hibiscus and offer a great potential for mass propagation of the ornamental shrub.

Technical Abstract: The regeneration ability of callus derived from three different plant fragments of eastern hibiscus (Hibiscus syriacus) was investigated. Callus was initiated from three different, 1-month-old seedling fragments (root, hypocotyls, and cotyledon) on Murashige-Skoog medium supplemented with 2,4-D or NAA, and BA, 2iP. The highest number of shoots, leaves, and roots was achieved from callus induced on NAA and BA medium and regenerated on medium with 0.1 mg liter1 of BA, or kinetin. The largest mass of callus was observed on medium containing 2,4-D and 2iP.