|Kappes, Steven - Steve|
Submitted to: Genome Research
Publication Type: Peer reviewed journal
Publication Acceptance Date: 8/11/2004
Publication Date: 10/1/2004
Citation: Ihara, N., Takasuga, A., Mizoshita, K., Takeda, H., Sugimoto, M., Mizoguchi, Y., Hirano, T., Itoh, T., Watanabe, T., Reed, K.M., Snelling, W.M., Kappes, S.M., Beattie, C.W., Bennett, G.L., Sugimoto, Y. 2004. A comprehensive genetic map of the cattle genome based on 3802 microsatellites. Genome Research 14:1987-1998. Interpretive Summary: Genetic linkage maps are used to track inheritance in pedigreed animals, to narrow the location of genes, and as foundations for connecting with other types of genetic maps. The marker density of an existing linkage map for cattle was substantially improved through an international collaboration. New microsatellite markers were identified and genotyped in families of cattle used for the previous linkage map. The number of markers on the new cattle linkage map more than doubled those on the earlier map to nearly 4,000. The new map will allow more choice of markers to track inheritance in cattle and should accelerate the discovery of genes affecting important traits in cattle.
Technical Abstract: A microsatellite-based high-density genetic map facilitates fine mapping of hereditary traits of interest, characterization of meiosis, and provides a foundation for physical map construction. A comprehensive genetic map was developed based on more than 880,000 genotypes across the USDA MARC cattle reference families with a potential genetic resolution of 0.8 cM at the 95% confidence level (about 800 kb in the bovine genome). We incorporated 2,325 microsatellites into the second-generation genetic map by linkage analysis based on sex-averaged two-point LOD scores greater than 3.0, of which 2,293 were fine-mapped by multipoint linkage analysis. The new 3,160-cM map of 29 sex-averaged autosomal linkage groups and a sex-specific X-chromosome linkage group includes 3,960 markers with 2,389 positions, resulting in an average interval size of 1.4 cM. More than half (51%) of the total length of the map is covered with intervals of 2.0 cM or less, and the largest gap is a 10.2-cM interval on the X-linkage group. The new map should accelerate fine mapping and positional cloning of genes for genetic diseases and economically important traits in cattle, as well as related livestock species, such as sheep and goat.