Submitted to: International Cotton Genome Initiative Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 10/10/2004
Publication Date: 10/10/2004
Citation: Stelly, D.M., Gao, W., Todd, S.Z., Chen, J., Yu, J. 2004. Wide-cross whole-genome radiation hybrid (WWRH) resources for cotton genomics [abstract]. International Cotton Genome Initiative Workshop. p. 25.
Technical Abstract: Genome maps are important to genome analysis, genetic manipulation, cloning and genetic engineering. Physical and linkage mapping methods are mutually complementary. Due to significant limitations of linkage maps alone, radiation hybrid (RH) mapping has been used extensively for physical mapping of human and animal genomes. However, RH mapping has been used very little in plants. We have developed a simple alternative approach that uses a wide cross in combination with whole-genome radiation to produce wide-cross whole-genome radiation hybrids (WWRH) that can be used for map construction. We report preliminary test results from cotton, where the genome of one species, Gossypium barbadense, was used to rescue radiation-segmented chromosomes of another species, G. hirsutum. Mature pollen grains from G. hirsutum were gamma-irradiated and used to pollinate emasculated G. barbadense flowers to generate nonchimeric WWRHs between those two species. A 5-Krad gamma ray WWRH mapping population was constructed and used to assemble a map. Results were compared to linkage maps, and further tested against cytogenetic stock data. A second generation WWRH panel was constructed and used to create another map, which was tested against the first map. The results indicate that WWRH mapping is robust and complements traditional linkage mapping, and that it will be useful for genomic analysis of cotton and perhaps other plant species. Plans are to provide these materials to the cotton research community to facilitate communication among laboratories and assembly of integrated resources. Toward those goals, collaborators and ideas are solicited from ICGI mapping groups. We gratefully acknowledge support from Texas Agric. Experiment Station, the TAMU Nuclear Science Center, and the Texas Advanced Technology Program.