Submitted to: International Cotton Genome Initiative Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 10/10/2004
Publication Date: 10/10/2004
Citation: Kohel, R.J., Yu, J. 2004. Permanent recombinant inbred mapping population for cotton genome research [abstract]. International Cotton Genome Initiative Workshop. p. 44.
Technical Abstract: The lack of a permanent mapping population in cotton (Gossypium spp.) hinders progress of many independent genetic studies that otherwise could be coordinated more effectively. We report on the characterization of 191 cotton recombinant inbred lines (RILs) that were derived by single seed decent from an interspecific F2 hybrid between TM-1 and 3-79, two genetic standards of G. hirsutum and G. barbadense, respectively. At F7 generation on average, this mapping population retains the phenotypic variability of the original F2, including fiber properties and other morphological traits. Approximately 200 cotton microsatellite or simple sequence repeat (SSR) markers were used to genotype the 191 RILs. Genetic segregation and allele distribution were assessed to provide a genomic evaluation for expanded uses of this permanent mapping population that is distributed (in DNA stocks) as a reference population to the international cotton research community. Linkage relations of the SSRs and graphical genotypes of the RILs facilitate further genomic characterization of this population that is at the foundation of internationally collaborated efforts to develop a high-density integrated map of the cotton genome and a basis for germplasm characterization.