Submitted to: Joint Abstracts of the American Dairy Science and Society of Animal Science
Publication Type: Abstract only
Publication Acceptance Date: 11/5/2004
Publication Date: 3/21/2005
Citation: Kim, J.W., Zhao, S.H., Uthe, J.J., Bearson, S.M., Tuggle, C.K. 2005. Physical mapping of five pig genes whose expression level is acutely affected by Salmonella challenge [abstract]. Joint Abstracts of the American Dairy Science and Society of Animal Science Midwest Meeting. p. 9. Interpretive Summary:
Technical Abstract: Infectious diseases increase the cost of production in the pig industry. Disease resistance has genetic heritability, and breeders are interested in raising productive pig breeds with increased resistance to disease. The objective of this research was to map genes identified as having altered gene expression patterns within the mesenteric lymph node during infection in a Salmonella enterica serotype Choleraesuis challenge. These five genes (DNAJA4, HSP90, HSP105, CXCR4, and IP-10) responded to infection within 48 hours of exposure. PCR primers were designed using Oligo 5.0 software. The primers of these genes amplified products only from pig genomic DNA, not mouse or hamster DNA. These genes were assigned to cytogenetic locations by using the INRA Somatic Cell Hybrid Panel and to precise physical linkage groups by using the IMpRH mapping panel. DNAJA4 was mapped to SSC7q12-q23 corresponding to HSA15q25.1, HSP90 was located on SSC7q26 (HSA14q32.33), HSP105 was mapped to SSC11p13 (HSA13q12.3), CXCR4 was located on SSC15q12-q14 (HSA4q21) and IP-10 was mapped to SSC8q11-q12 (HSA4q21). For the RH mapping, the retention fraction for each gene was 0.23 (DNAJA4), 0.30 (HSP90), 0.27 (HSP105), 0.38 (CXCR4), and 0.35 (IP-10). The highest Lod score between DNAJA4, HSP90, HSP105, CXCR4, and IP-10 and the nearest locus was 7.23, 5.77, 7.13, 14.06, and 18.43, respectively. All physical mapping data was internally consistent, and the predicted location in pig based on human:pig comparative maps was confirmed by mapping data. These data also confirm the functional annotation of these sequences. The PCR amplicons can be used to develop SNPs at these expression candidate genes and to determine the association of these genes with variation in challenge response. Such associated markers can be used to develop pigs with improved resistance to bacterial diseases. J.W. Kim is recipient of a KOSEF Post-doctoral Fellowship Program.