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ARS Home » Pacific West Area » Corvallis, Oregon » National Clonal Germplasm Repository » Research » Publications at this Location » Publication #168426
Title: COLD STORAGE OF IN VITRO APPLE IN CONDITIONS OF SLOW GROWTH

Author
item KOVALCHUK, I. - KAZAKH INST. OF POMOLOGY
item Reed, Barbara

Submitted to: Perspectives in Kazakhstan Agricultural Ministry, Astana
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/20/2004
Publication Date: N/A
Citation: N/A

Interpretive Summary: Apple tissue culture plants were tested for the ability to withstand refrigerated storage. The plants in test tubes or plastic storage bags were placed in the chamber with refrigerator temperatures,low light intensity and 10 hours of light. Growth regulators, carbohydrates and volume of storage containers were studied with the apple cultivar Grushovka Verninskaya and a wild apple TM-6. The sugars sucrose and mannitol were tested in small and large containers. The best plant condition was observed on the standard apple medium containing hormones and 3% sucrose. Good results were also seen for plants on medium without hormones containing 3% sucrose. The volume of the vessel was not important if the growth medium contained hormones, but for cultures without hormones the larger container with sucrose or a mixture of sucrose and mannitol were best.

Technical Abstract: Apple in-vitro plants were tested for the ability to withstand refrigerated storage. The microplants in test tubes or plastic storage bags were placed in the chamber with low temperatures (5 '/ 45 F),low light intensity (7 'mol m-2s-1) and a 10 hour photoperiod. Growth regulators, carbohydrates and volume of storage vessels were studied with the apple cultivar Grushovka Verninskaya and a wild apple TM-6. The basic growth medium was MS. 6-benzylaminopurine (BAP), indole-3-butyric acid (IBA), abscisic acid (ABA) were used as the growth regulators. Carbohydrates ' sucrose and mannitol were used. Vessels with 130 ml volume and 10 ml medium and test tubes with 55 ml volume and10 ml medium were tested. The best plant condition was observed on the standard apple proliferation MS medium, containing hormones and 3% sucrose. Good results were also seen for plants on medium without hormones containing 3% sucrose and in medium with 1% abscisic acid +3% sucrose. The volume of the vessel was not important if the growth medium contained hormones, but for cultures without hormones the 130 ml volume with 3% sucrose and 2% sucrose + 2% mannitol was best.