|Kappes, Steven - Steve|
Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/28/2003
Publication Date: 12/23/2003
Citation: Ihara, N., Yamakuchi, H., Taniguchi, Y., Sasaki, Y., Bennett, G.L., Kappes, S.M., Sugimoto, Y. 2003. Mapping of bovine CEBPD gene to BTA14q15-17. Animal Genetics. 34:470-471. Interpretive Summary: The chromosomal location of cattle genes that might be involved with physiological processes is important information for finding genetic differences in economically important traits. A particular gene called CEBPD that may regulate how fat-specific genes are expressed and therefore influence body fat composition and distribution was located on cattle chromosome 14. This work adds to the accumulating number of genes that may affect important traits, have been located on cattle chromosomes, and correspond to genes identified in other species. This information will be important for researchers trying to identify the genetic basis for economic differences in cattle.
Technical Abstract: CCAAT/enhancer-binding proteins (CEBPs) and peroxisome proliferators activated receptor c (PPARG) are transcription factors that regulate the expression of fat-specific genes, and have been reported to control differentiation of mouse preadipocyte cell lines. These bovine orthologs may influence body fat composition and distribution, which are economically important traits in beef cattle. Bovine CEBPA and PPARG genes have been previously mapped to BTA18q24 and BTA22q24, respectively. Here the bovine CEBPD gene is mapped to BTA14q1517 by fluorescence in situ hybridization (FISH) using a yeast artificial chromosome (YAC) clone harboring the gene, and by genetic linkage mapping of a polymorphic microsatellite marker isolated from the YAC clone. Using FISH, target DNA was hybridized to BTA14q1517 corresponding to HSA8q11 where the human CEBPD gene is located. Heterozygosity of DIK121 was 0.62 across 28 parents in the USDA-MARC mapping population, with identification of seven alleles and a product size of 189201 bp. Using CRIMAP, DIK121 was linked to RM180 and RM011 on BTA14. Thus there is a good agreement between the physical assignment of CEBPD gene by FISH and genetic localization by linkage analysis.