Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/22/2004
Publication Date: 8/22/2004
Citation: Manners, G.D., Jacob, R.A., Breksa III, A.P., Schoch, T.K., Hasegawa, S. 2004. Bioavailability of citrus limonoids in humans. 2004 Meeting Abstract. ACS Symposium, August 2004, Philadelphia, PA. Interpretive Summary:
Technical Abstract: Citrus limonoids have demonstrated significant anti-tumor activity in animal and cancer cell screens. In this study we examined the plasma of four groups of four healthy male and female subjects (nine women and seven men, 19-51 yrs) and administered pharmacological doses (0.533, 1.06, 2.12 and 4.24 mmol) of pure limonin glucoside for the presence of the limonoid aglycone limonin to establish the metabolism and bioavailability of citrus limonoids in humans. LC-MS analysis of the plasma of the study subjects revealed increasing amounts of limonin associated with administered dose in 0.533, 1.06 and 2.12 mmol subject groups. The time to maximum concentration was 6 h in 13 of 16 subjects. The results of this study represent the first confirmation of the metabolism and bioavailability of limonoids in humans and establishes that citrus limonoids may play an important role in the benefits of citrus to human health and nutrition.