|Harrison, Melanie - Newman, Melanie L|
Submitted to: Biomed Central (BMC) Plant Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/24/2003
Publication Date: 4/24/2003
Citation: He, G., Meng, R., Newman, M.L. aka Harrison Dunn, M.L., Gao, G., Pittman, R.N., Prakash, C.S. 2003. Microsatellites as dna markers in cultivated peanut (arachis hypogaea l.). Biomed Central (BMC) Plant Biology. Biomed Central Plant Biology (BMC) 2003; 3:3 Interpretive Summary: The cultivated peanut, Arachis hypogaea, is a major agricultural crop that is used for both its edible fruit and its high quality oil. Traditional breeding efforts to improve various traits of the peanut, including oil, quality, nutritional quality, disease/pest resistance, increased yield and reduced allergenicity, could be much more efficient and successful with the use of molecular markers. This study has identified 19 molecular markers that can be used by breeders in improving the peanut and also has demonstrated that a procedure, called simple sequence repeat (SSR) enrichment, is a useful tool in developing even more markers in the peanut.
Technical Abstract: Genomic research of cultivated peanut has lagged behind other crop species because of the paucity of polymorphic DNA markers found in this crop. It is necessary to identify additional DNA markers for further genetic research in peanut. Microsatellite markers in cultivated peanut were developed using the SSR enrichment procedure. The results showed that the GA/CT repeat was the most frequently dispersed microsatellite in peanut. The primer pairs were designed for 56 different microsatellites, 19 of which showed a polymorphism among the genotypes studied. The average number of alleles per locus was 4.25 and up to 14 alleles were found at one locus. This suggests that microsatellite DNA markers produce a higher level of DNA polymorphism than other DNA markers in cultivated peanut. It is desirable to isolate and characterize more DNA markers in cultivated peanut for more productive genomic studies, such as genetic mapping, marker-assisted selection, and gene discovery. The development of microsatellite markers holds a promise for such studies.